Composite
Part:BBa_K3409012:Design
Designed by: Albane Mabro Group: iGEM20_Ionis_Paris (2020-10-17)
Microcin PDI cluster gene for McpB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Used the Salis Lab Algorithm to predict the Translation Initiation Rates of the start codon (according to different combinations of Promoters and Ribosome Binding Sites) to make sure the mRNA was translated in similar levels to the native mcpB gene from the cluster. Only contains an RBS because meant to be downstream of BBa_K3409011. Synthesis provided by IDT. The presence of all 5 genes from the cluster are essential for appropriate expression and secretion of Microcin PDI.
Source
This is a composite part including the mcpB coding region encoded in BBa_K3409007.